Analysis of Genetic Parameters in Order to Get More Information on High Producing Recombinant CHO Cell Lines

Recombinant mammalian cells for the production of glycoproteins and antibodies are mainly established by gene amplification techniques, using selection and screening methods based on specific productivity and growth rate. In the present study we will show the selection and screening process of a recombinant CHO cell line expressing a Fc fusion protein by analysis of gene copy numbers, mRNA-levels, specific productivity and integration locus.