Novel virus maintenance solution for preparation of recombinant avian influenza virus H5N1 antigen

2015 
The invention belongs to the technical field of biological engineering, and in particular relates to a novel virus maintenance solution for preparation of a recombinant avian influenza virus H5N1 antigen. The virus maintenance solution comprises the following components: 6.5g of DMEM (high-sugar) dry powder medium, 5.0g of DMEM (low-sugar) dry powder medium, 1,000ml of injection water, 20mM of Hepes and 2mg of a TPCK configuration solution. The TPCK configuration solution with the specification of 2mg/ml are prepared by adding 10g of TPCK dry powder into 50 ml of a PBS solution of which a pH value is 7.2; the PBS solution is prepared from 8.0g of sodium chloride, 0.2g of potassium chloride, 1.25g of disodium hydrogen phosphate, 0.2g of monopotassium phosphate and 1,000ml of injection water. The novel virus maintenance solution is mainly used in a preparation process of the recombinant avian influenza virus H5N1 antigen.
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