Collection of Lymphocytes on a Continuous‐Flow Blood Cell Separator

. Lymphocytes have been collected by continuous flow leukapheresis from cancer patients (pre-treatment) and stored at -190 °C in liquid nitrogen as immunological support for intensive chemotherapy. Described here are methods for obtaining maximum yield and viability. Assessments are made of cell count, both during and after leukapheresis, and of viability just prior to storage.