Antioxidant effect of Moringa oleifera leaves in hemoglobin oxidation compare with vitamin C.

Objective The aim of this study is to investigated the effect of MO leaves extract as an antioxidant in inhibiting methemoglobin (MetHb) formation compared with Vitamin C as positive control. Methods This study was an experimental study in vitro comparing the antioxidant effects of MO leaves extract with vitamin C as positive control and saline as a negative control. Hemoglobin was oxidized with potassium ferrocyanide then the antioxidant effect of MO leaf extract was determined by measuring methemoglobin levels. Results This study showed that MO leaves extract have antioxidant effects compared to saline, MO leaves extract 1600ppm showed no significant differences with Vitamin C 1600ppm (p=0.067). MO leaves extract 3200ppm showed no significant difference with all Vitamin C levels (p>0.05). This shows that MO leaves extract has antioxidant effects like Vitamin C. Conclusion M. oleifera leaves extract has antioxidant effects on hemoglobin oxidation induced by potassium ferricyanida.