Identification of the interaction between classical swine fever virus E2 protein and porcine RACK1

To screen and identify the porcine proteins interacted with classical swine fever virus(CSFV) E2 protein,a potential interacting partner of RACK1 was screened with CSFV E2 as bait protein by yeast two-hybrid from porcine alveolar macrophage expression library.The interaction of the two proteins was further confirmed by co-transformation,GST pull-down and laser confocal assays.These results proved that CSFV E2 interacted with RACK1.