Establishment of a new model of secondary intra-abdominal hypertension in rats

Objective To create a stable,reliable and cheap rat model of intra-abdominal hypertension(IAH)by simulating the development of secondary IAH after overdose resuscitation for severe hemorrhagic shock.Methods Sixteen adult male SD rats were randomly divided into sham group and portal vein stenosis group,with eight rats in each group.Portal vein stenosis group had transabdominal separation of portal vein with constant stenosis,while sham group underwent simple laparotomy and dissection of portal vein.Abdominal binding and hemorrhagic shock by drawing blood through the femoral artery cannulation with mean arterial pressure(MAP)of 40 mm Hg(1 mm Hg=0.133 kPa)for two hours were performed in two groups.Then,reinfusion of all autologous blood and continuous infusion of sodium lactate Ringer's solution were performed to induce IAH.Results In the portal vein stenosis group,IAH models were successfully built in all the rats except that one rat died after ligation of portal vein.The success rate of modeling was 87.5％,with total time of(4.68±0.53)hours and total solution infusion of(597.33±75.03)ml/kg.No successful modeling was obtained in the sham group.Conclusion High success rate and good reproducibility of IAH models can be achieved by fluid resuscitation after constant portal vein stenosis,abdominal binding and hemorrhagic shock with mean MAP of 40 mm Hg for two hours. Key words: Abdominal cavity; Main portal vein; Shock,hemorrhagic; Resuscitation; Rats