Single-Molecule Force Spectroscopy Measures Structural Changes Induced by Light Activation and Transducer Binding in Sensory Rhodopsin II

Abstract Microbial rhodopsins are a family of seven-helical transmembrane proteins containing retinal as chromophore. Sensory rhodopsin II (SRII) triggers two very different responses upon light excitation, depending on the presence or the absence of its cognate transducer HtrII: Whereas light activation of the Np SRII/ Np HtrII complex activates a signalling cascade that initiates the photophobic response, Np SRII alone acts as a proton pump. Using single-molecule force spectroscopy, we analysed the stability of Np SRII and its complex with the transducer in the dark and under illumination. By improving force spectroscopic data analysis, we were able to reveal the localisation of occurring forces within the protein chain with a resolution of about six amino acids. Distinct regions in helices G and F were affected differently, depending on the experimental conditions. The results are generally in line with previous data on the molecular stability of Np SRII. Interestingly, new interaction sites were identified upon light activation, whose functional importance is discussed in detail.