Improving the Lipase Production of Pseudomonas fluorescence 26-2 through Transform Its Lipase Gene into the Originally Strain

A Pseudomonas fluorescence lipase gene lipA was subcloned from ppic9k-lipA and inserted into vector pDSK519 to construct a new plasmid,pDSK519-lipA,which was transformed into its original strain P.fluorescence 26-2.The recombinant strain 26-2-1 was confirmed by restriction digestion.Compared with wild type 26-2 in pot experiment and ferment experiment,the lipase production of 26-2-1 is 2 times more than its original strain