Membrane Protein Crystallization Mediated by Antibody Fragments

Originally published in: Molecular Biology in Medicinal Chemistry. Edited by Theodor Dingermann, Dieter Steinhilber and Gerd Folkers. Copyright © 2004 Wiley-VCH Verlag GmbH & Co. KGaA Weinheim. Print ISBN: 3-527-30431-8 The sections in this article are Introduction Crystallization of Membrane Proteins Mediated by Antibody Fragments Overview Generation of Antibodies Suitable for Co-crystallization Immunization Selection of Antibodies Antibody Fragments Protein Purification and Crystallization Indirect Immunoaffinity Purification Crystallization Alternative Approaches for Expansion of the Polar Surface Conclusions Acknowledgments Keywords: molecular biology; medicinal chemistry; analysis; antibody fragments as crystallization enhancers; crystallization of membrane proteins