Effects of streptolysin O, polyriboinosinic-polyribocytidylic acid and combinations with cyclophosphamide on the hepatic arylamine N-acetyltransferase and cytochrome P450-dependent monooxygenases in rats.

There is evidence that immunostimulation depresses the functions of various cytochrome p450 (CYP)-dependent monooxygenases but activates the arylamine N-acetyltransferase (NAT). Therefore, the effects of the synthetic immunostimulator polyriboinosinic-polyribocytidylic acid (plC, 8mg/kg i.p.), or sublytic doses of streptococcal streptolysin O (SLO, 50HU/kg i.V; for 5d) and the immunosuppressor cyclophosphamide (CP, 100mg/kg i.p.) on NAT and some monooxygenases were studied in rat liver. It was also evaluated whether CP might antagonize the effects of plC and SLO on drug metabolism. SLO, plC and CP reduced CYP content and the activities of some mooxygenases. NAT was significantly inhibited by CP (given 5d before sacrifice) but not by plC, SLO or CP when given 2d before sacrifice. CP lacked any effect on NAT if it was administred prior to SLO. However, it deteriorated synergistically the inhibition of the monooxygenases caused by SLO and pLC