Opioid receptor binding in CXBK mice brain

It has been reported that the CXBK strain mouse is deficient in μ1 type opioid receptor. The properties of opioid receptor binding in CXBK were compared with those of C57BL/6, progenitor mouse strain of CXBK. In the membrane preparations from whole brain, CXBK demonstrated a small Bmax value for 3 H-DAGO binding and a large Kd value for 3 H-U-69593 binding, compared with those of C57BW6. In all brain regions of CXBK, except cerebellum, the 3 H-DAGO binding showed lower values. The CXBK strain exhibited a significant decrease in the 3 H-U-69593 and 3 H-DPDPE-Cl binding to striatum