Nucleotide sequences of the genes encoding fructosebisphosphatase and phosphoribulokinase from Xanthobacter flavus H4-14

SUMMARY: The genes encoding fructosebisphosphatase and phosphoribulokinase present on a 2·5 kb SaiI fragment from Xanthobacter flavus H4--14 were sequenced. Two large open reading frames (ORFs) were identified, preceded by plausible ribosome-binding sites. The ORFs were transcribed in the same direction and were separated by 39 base pairs. They encoded proteins of 364 and 291 amino acids, with molecular masses of 38739 and 33409 Da, respectively. The ORFs were identified as the genes encoding FBPase and PRK, respectively, on the basis of similarity with FBPase and PRK sequences from other sources.