Validation of Genotyping Protocols for Diagnostic Use

This chapter contains some advice for fi rst validating a new genotyping method and then continuing to ensure that it performs well when adopted for routine use. It should be applicable to most of the genotyping methods described in this volume; however, because the range of techniques is so wide and these recommendations have been written with PCR amplifi cation in mind, the reader may need to adapt some of the recommendations for non-PCR based tests. It does not include any advice on how to handle unscreened human samples within the laboratory, and it assumes that the laboratory already has established good practice for DNA amplifi cation and the avoidance of contamination.