Biophysical approaches determining ligand binding to biomolecular targets : detection, measurement and modelling

Introduction: The who, where, why questions NMT X-ray Surface plasmon resonance Flourescence Electropspray ionization mass spectrometry Synchrotron circular dicroism Thermal denaturation in presence of a fluorescent dye Calorimetry Molecular modelling Examples of the simultaneous use of several techniques to determine ligand binding