Characterisation of highly ordered pyrolytic graphite with covalently attached ferritin by electron probe microanalysis

Ferritin has been covalently attached to highly ordered pyrolytic graphite using cyanuric chloride as the linking agent. Scanning electron microscopy and energy-dispersive analysis were used to examine the surface morphology after each step in the modification and to determine the amount of ferritin bound to the edge and basal planes of the highly ordered pyrolytic graphite. The concentration of ferritin found on the edge plane was three times higher than that found on the basal plane.