Structure and function changes in rat adrenal glands during aging

The current study examines corticosterone production in young and old rats with a view to understanding how hormone production is related to aging changes in the adrenal gland. For this purpose, we compared total (plasma volume-corrected) values for adrenocorticotropic hormone (ACTH)-induced maximal corticosterone production in young, mature (5-mo-old), and aging (18-mo-old) rats. These values were subsequently corrected for measured age-related differences in adrenal weight, adrenal cortex volume, specific adrenal zone volumes, and total number of corticosterone-producing cells in the adrenals of the differently aged rats. In addition, corticosterone disposal rates were measured in the two groups of rats, and adrenal cortical ACTH binding sites were identified by autoradiography after perfusion with 125I-labeled ACTH analogue. The results show that maximal serum ACTH-induced corticosterone concentrations (per ml serum) in the 18-mo-old rats are somewhat less than those seen in the younger animals. However, because the plasma volume is greater in the older animals (and corticosterone disposal rates do not vary), total circulating corticosterone production is, in fact, equivalent in the two groups of animals. When these total values for corticosterone are corrected for various structural changes in the adrenal gland with age, corticosterone production was found to be lower in the older group of rats: i.e., one sees an approximately 50% decline when corticosterone is expressed per adrenal weight or zonal volume and an approximately 20% decline when expressed per number of endocrine cells per adrenal.(ABSTRACT TRUNCATED AT 250 WORDS)