Preparation method and application method of charged nanostructured cell chip

The invention relates to a preparation method and an application method of a charged nanostructured cell chip used for high efficiency detection of cells, and belongs to the technical fields of functional materials, biomedical materials and detection analysis. The preparation method comprises the following steps: placing a clean transparent substrate on flame, carrying out physical deposition on the surface of the substrate to obtain ash layer composed of nanometer ash particles, carrying out water vapor deposition on the ash substrate, carrying out chemical vapor deposition by adopting a silicon-containing compound with the obtained substrate as a template, and depositing a silica shell layer on the surface of ash particles; carrying out high temperature calcining, and removing nanometer ash particles to obtain a nanostructured silica layer; and modifying the surface of the silica layer with a chemical reagent containing an amino group or a carboxyl group to obtain the charged nanostructured cell chip used for cell detection. The charged cell chip has the advantages of simple preparation method, good optical performances, high capture rate and high adhesion rate.