DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR THE ASSAY OF TRAMADOL HCl IN ITS CAPSULE FORMULATION

A simple, new, specific, sensitive, rapid, and economical procedure has been developed for the assay of Tramadol HCl in its capsule formulation. The method is based on the measurement of ultraviolet absorbance maxima of the above drug at 271nm using 0.1N Acetic acid as diluent, which does not shows any interference in spectrophotometric estimations. Beer’s law was obeyed in concentration range 0-130mcg/ml having line equation y = 0.00683 x + 0.01167 with correlation coefficient value 0.99953. The drug obeyed Beer's law in the concentration range of 5 – 130 mcg/ml. All the parameters of the analysis were chosen according to ICH [Q2 (R1)] guidelines and validated statistically.