Coupling Electrochemical Adsorption and Long‐range Electron Transfer: Label‐free DNA Mismatch Detection with Ultramicroelectrode (UME)

A new electrochemical hybridization trans-duction pathway, obtained by coupling electrochemical adsorption and long-range electron transfer through double-stranded DNA, was investigated using ultrami-croelectrode (UME). The results show that long-range electron transfer does not occurs exclusively throws well-packed and organized self-assembled DNA monolayers. This approach is used to investigate long-range electron transfer properties of both single-and double-stranded short synthetic DNA and DNA plasmids. Single mismatch electrochemical detection protocol of non-labelled short synthetic DNA, without heating or probe labelling, in a 10 minutes protocol, was in fine performed.