A Validated RP-HPLC Method for Estimation of Metoclopramid e Hydrochloride and Paracetamol in solid dosage form

A simple, specific, accurate, precise and reproducible method has been developed and validated for the simultaneous estimation of Metoclopramide hydrochloride and Paracetamol in combined dosage form by RP-HPLC method. RP-HPLC estimation of drugs in selected combination was done using Phenomenex ODS 5µ, C 18 column (250×4.6mm) and ACN: Methanol: (0.5%) TEA Buffer (18.5:6.5:75) as mobile phase which shows sharp and resolved peak when detected at 273nm. The linearity range was found to be in concentration range 10-50 µg/mL for Metoclopramide (MET) and 10-50 µg/mL for Paracetamol (PAR). The retention time for Paracetamol and Metoclopramide were 4.1 and 5.9 respectively. The correlation coefficient was found to be 0.9999 (for MET) and 0.9992 (for PAR). The mean percentage recovery was found to be 98.69 and 98.92 for MET and PAR respectively. The % estimation of the drugs was found near to 100 % representing the accuracy of the method. Validation of the proposed method was carried out for its accuracy, precision, specificity and ruggedness according to ICH guidelines. The proposed method can be successfully applied in routine work for the determination of Metoclopramide and Paracetamol in combined dosage form.