Relationship between nutrition factors and osteopenia: Effects of experimental diets on immature bone quality

Abstract To investigate the influence of experimental diets on morphological and mechanical characteristics of immature bone, this study thoroughly examined the nutrition–bone connection. A non-destructive evaluation method involving high-resolution in-vivo micro-computed tomography and finite element (FE) analysis was used to investigate the relationship between obesity and osteopenia—two disorders of body composition. Correlation of nutritional deficiency with bone characteristics was also investigated. Some recent studies have shown that both obesity and osteopenia share several common genetic and environmental factors. However, there have been few studies correlating these pathologies in-vivo from a structural and biomechanical point of view. In the present study, detailed changes in morphological and mechanical characteristics of trabecular bone architecture were detected and tracked by longitudinal studies of morphometric parameters and simulated compression testing. Rats were randomized into three groups: overeaten diet (OD) for formation of obesity, normal diet (ND), and restricted diet (RD) in which rats received 65% of the normal diet. In the OD and ND groups, all structural parameters changed significantly ( p p p p >0.05). The key finding of the present study is that fat mass is morphologically and mechanically inversely correlated with bone mass when the mechanical loading effects of greater body weight on bone mass are applied.