Simple method for DNA isolation from urediospore of wheat leaf rust pathotypes

*Email a-abasi@Yahoo.com Genetic analysis of obligate parasites like rust pathogens is difficult and sometimes impossible to multiply the isolates on synthetic medium (6). DNA separation directly from urediospore, multiplied on living host is promising. Existing procedures for isolation of DNA from wheat leaf rust pathotypes yielded little amount of DNA from large amount of urediospore. Therefore, in the present study, a simple protocol was developed for DNA isolation from urediospore and RAPD analysis for different pathotypes of Puccinia recondita tritici.