A MICROFLUIDICS-BASED MICROCYTOMETER: INTERFACING MICROFLUIDICS WITH MACROFLUIDICS

We have developed an instrument for performing cytomechanical studies of red cell membrane viscoelastic behavior during flow in microfluidic channels of circa (3×3)μm section. This paper discusses interfacing such structures to the necessary supporting ‘millifluidic’ systems that provide operational functionality. Such microfluidic interfacing is more complex in the case of particle-carrying suspensions due to sedimentation and separation effects. Cell-carrying suspensions are additionally characterized by the relative fragility of cells and resulting operational constraints in sample handling. A system and matching microfluid device that seek to address these limitations are presented here. The results of preliminary trials are presented showing broad agreement with design specifications.