Establishment of Activity Detection Method of Calf Intestine Alkaline Phosphatase

[Objective] The research aimed to establish the detection method of the activity of alkaline phosphatise for scientific research.[Method]The using conditions and buffer of calf intestine alkaline phosphatise in the scientific research were simulated and the effects of different substrate concentration,reaction time,coloration time,buffer pH and other factors on the detection of enzyme activity were studied.[Result]Different kinds of buffer had greater effects on the activity of alkaline phosphatise. When potassium ferricyanide solution with adding boracic acid was used,the coloration was stable. The best time of water bath was 10 min and the best substrate concentration was 0. 04 mol /L.As the temperature increased,its effects on the activity of alkaline phosphatise at lower concentrations was little,but its effects on the activity of alkaline phosphatise at higher concentrations were greater. When the pH of buffer was 7. 0 ~ 8. 0,the stability of the activity of alkaline phosphatise could be maintained. The granule intensity of buffer had little effects on the activity of alkaline phosphatise. [Conclusion]The detection method of the activity of calf intestine alkaline phosphatase for scientific research was successfully established.