Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry
2018
In this study, the microscale thermophoresis (MST) method was
applied to investigate the interaction of deferiprone with
biometals (Fe3+, Cu2+, Zn2+, Co2+, Ni2+, Mn2+, Mg2+, and Ca2+).
The MST results indicated significant interactions of
deferiprone with Fe3+, Cu2+, Zn2+, Co2+, and Ni2+. The data
fitted to the Hill model shows stoichiometry more than 1:1. No
binding was observed for deferiprone with Mn2+, Mg2+, and Ca2+.
Electrospray ionization mass spectrometry confirms the results
obtained by MST indicating stable molecular ion peaks for the
complexes of deferiprone with either Fe3+, Zn2+, Cu2+, Co2+,
Ni2+, and Mn2+, but no binding was observed for deferiprone
with either Ca2+ and Mg2+ under a gas phase state. MST shows a
fast and simple approach to study the binding of deferiprone to
different essential metal ions. Moreover, the complexes were
stable for investigation by mass spectrometry under a soft
ionization technique like used electrospray ionization, which
aided to confirm binding stoichiometry.
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