Engineering of weak helper interactions for high-efficiency FRET probes

Raik Grünberg,Julia Burnier,Tony Ferrar,Violeta Beltran-Sastre,François Stricher,Almer M. van der Sloot,Raquel Garcia-Olivas,Arrate Mallabiabarrena,Xavier Sanjuan,Timo Zimmermann,Luis Serrano

Engineering of weak helper interactions for high-efficiency FRET probes

2013

Raik Grünberg
Julia Burnier
Tony Ferrar
Violeta Beltran-Sastre
François Stricher
Almer M. van der Sloot
Raquel Garcia-Olivas
Arrate Mallabiabarrena
Xavier Sanjuan
Timo Zimmermann
Luis Serrano

Addition of weak helper interactions to fluorescent protein pairs by protein engineering provides a simple method to increase FRET efficiency with little or no background.

Keywords:

Protein engineering
Cellular imaging
Förster resonance energy transfer
Fluorescence-lifetime imaging microscopy
Fluorescence
Protein design
Bioinformatics
Biology
fluorescent protein

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