Early increase in glomerular leucocyte number after a reducton in renal mass : implications for the pathogenesis of glomerulosclerosis
1993
1. Adult female Wistar rats underwent uninephrectomy (n=8) through a flank incision, or a sham operation (n=7). One to two weeks later the kidney was perfused in situ and glomeruli were isolated from cortical tissue by sequential sieving, and partially digested. Glomerular leucocytes were labelled with a mouse monoclonal antibody against leucocyte common antigen followed by a fluorescein-labelled anti-mouse immunoglobulin to allow counting. 2. In a further group of animals 24 h albumin excretion and glomerular size were measured 2 weeks after either uninephrectomy (n=6) or sham operation (n=6)
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