DETERMINATION ON CELL CULTURES OF THE PROLIFERATIVE ACTION OF SOME SEMISOLID PREPARATIONS FOR TOPICAL USE WITH AMYGDALI OLEUM

The objective of the study was to evaluate the the cellular proliferation and cell morphology of the gel with Amygdali Oleum var. dulcis (sweet almond oil) on cell cultures. The testing was performed using a line of human lung fibroblasts (MRC), cultivated on a Minimum Essential Medium (MEM) culture containing 10% fetal serum and antibiotics. The MTT test [(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)] for cellular proliferation was performed after 24 hours, 48 hours and 72 hours incubation of the cells in the presence of the tested substances. We tested semisolid preparations for topical use containing 5% Amygdali oleum. Cicatrizin® was used as reference. Cellular proliferation values increased in direct proportion with the cultivation time. At concentrations of 25 mg/mL and 50 mg/mL, the cellular proliferation was higher than for the 10 mg/mL concentration. The human fibroblasts proliferation rates in the presence of sweet almond gel and of Cicatrizin® were comparable with those of the control sample. Electronic microscopy images showed that the tested products did not induce morphological modifications of the cultured fibroblasts. After 72 hours of incubation, the cells maintained the phenotype and characteristics of normal fibroblasts. Rezumat Studiul a urmărit evaluarea acţiunii proliferative si a morfologiei celulare induse de gelul cu Amygdali Oleum var. dulcis (ulei de migdale dulci) in culturi de celule. Testarea a fost efectuată pe o linie de fibroblaste din plămân uman – MRC, cultivate in mediu de cultură MEM conţinând ser fetal 10% si antibiotice. Testul MTT de determinare a proliferării celulare a fost realizat după 24 de ore, 48 de ore si respectiv 72 de ore de incubare a celulelor in prezenţa substanţelor testate. Pentru testări s-au luat in lucru preparate semisolide pentru uz topic cu Amygdali Oleum in concentraţie 5%, comparativ cu Cicatrizinul® ca preparat semisolid de referinţă. Valorile proliferării celulare au crescut direct proporţional cu timpul de incubare la ambele probe. La concentraţii de 25 mg/mL si 50 mg/mL, gradul de proliferare celulară a fost mai mare decât la concentraţia de 10