Whole genome sequencing of Streptococcus pneumoniae serotype 33C causing fatal sepsis in a hospitalized patient with nephrotic syndrome

Abstract There are limited data on genome perceptions of S. pneumoniae using whole genome analysis of clinical strains originated from Iraq. Herein, we report the whole genome analysis of S. pneumoniae IMS597, serotype 33C a highly susceptible strain isolated from a hospitalized patient having nephrotic syndrome with sepsis. Whole genome sequencing of the isolate was performed by next-generation sequencing. Isolate IMS597 was susceptible to Penicillin, Erythromycin, Chloramphenicol, Cefotaxime and vancomycin. Serotyping by sequential multiplex PCR indicated its serotype 10F/10C/33C-F, and Quellung reaction confirmed it as 33C serotype. Multi-locus sequence typing (MLST) of strain IMS597 revealed that it belongs to the sequence type 1701. A multifaceted interaction of capsular type and the various virulence determinants particular to each serotype impact the ability of that strain to cause disease with varying severity.