Butylhydroxytoluene induce apoptosis to pulmonary macrophage in rat lung

Objective In order to study the apoptosis effect on pulmonary macrophage induced by butylhydroxytoluene(BHT). Methods 40 SD rats were divided randomly into three different doses groups of BHT and one group as menstruum control and one group as water control.The rats belonging to BHT groups were given separately by the dose of 300,600 and(1 200 mg/kg)(po),for 8 days.Results At the end of exposure,coefficient of lung was increased with the BHT dose raising.Pulmonary pathology determination showed that there were raised alveolus spacing and interstitial inflammation in middle and high BHT dose groups,but fibrosis were not found.BHT could induce apoptosis to pulmonary macrophage in the perfusate of isolate perfused rat lung as examined by the technology of PI fluorescence stain and comet assay.Conclusion BHT may cause functional and organic damage of lung and liver and it can induce apoptosis to pulmonary macrophagein rat.