for Surveillance of Measles Immunity High-Efficiency Mammalian Expression System Hemagglutinin Protein Produced in a Immunosorbent Assay Based on Recombinant

Recombinant hemagglutinin (H) protein of the measles virus (MV) was produced in mammalian cells witha high-yield expression system based on the Semliki Forest virus replicon. Crude membrane preparations ofH protein-transfected BHK-21 cells were used to coat microtiter plates to measure specific immunoglobulin Gantibodies in 228 serologically defined serum samples mainly from measles late-convalescent adults. The titersby the enzyme-linked immunosorbent assay for the H protein (H-ELISA) closely correlated with neutralizationtest (NT) titers (