Isolation of active constituents from Wagatea spicata using preparative HPTLC and structural elucidation using FTIR and NMR and GCMS techniques

Wagatea spicata (synonymously known as Moullava spicata) is a perennial plant of Leguminosae family having ethnomedical significance as a skin disease healer [1]. It exhibits a diverse concentration of biologically active constituents such as Lupeol, Bergenin, Stigmasterol, Friedelin, n-Hexadecanoic acid, Palmitic acid, Gamma sitosterol [2]. However the systematic study based on qualitative and quantitative detection of several marker compounds from Wagatea spicata is essential to reveal the entire phytochemical profile of the plant. Therefore, the primary objective of this work was to isolate and characterize the phytoconstituents from Wagatea spicata which may possibly be contributing to its biological activity. Additionally this will contribute towards establishment of quality of the aerial plant material. Preparative HPTLC method was developed for the rapid and less laborious, greener isolation of components from the methanolic leaf extracts of Wagatea spicata. Techniques such as Fourier Transform Infra Red Spectroscopy, Nuclear Magnetic Resonance Spectroscopy & Gas Chromatography Mass Spectrometry (GCMS) were employed for structural elucidation & identification of isolated components.