An immunochemical analysis of enzymatic hydrolysates of milk and soy proteins intended for the production of specialized nutritional products

: The capacities of immunoelectrophoresis in identification and assay of antigenic protein in enzymatic hydrolysates of milk and soya have been evaluated. The hydrolysates obtained under the action of pancreatin and protosubtilin were studied in milk protein concentrates "Dialact" and casezit and soya protein isolate "Adrex". Two-dimensional immunoelectrophoresis according to Clarke and Freeman was used to analyze the antigenic component spectrum in the samples. It has been shown that beta-lactoglobulin, as compared to other milk proteins, is most resistant to pancreatin-induced proteolysis in vitro. Pancreatin-induced proteolysis has been shown to be useful for trypsin inhibitor inactivation in soya protein isolate. It was demonstrated that immunoelectrophoresis could be used for complex evaluation of protein hydrolysates intended for manufacturing specialized foodstuffs.