Polymerase Chain Reaction Based Detection of Dolichos Yellow Mosaic Virus Infecting Dolichos

A survey was conducted at Vegetable Farm of Chandra Shekhar Azad University of Agriculture and Technology, Kanpur during Feb- March 2010 to know the occurrence of DoYMV. The infected sample showing characteristic yellow mosaic symptoms include faint chlorotic specks on leaf lamina, which later develop into bright yellow mosaic patches with small island of green tissue, mottling and leaf distortion were observed and collected to diagnose the causal virus. Four set of primers (DAC1-F 5’ GTG CGT GAA TCC GTC ATTTCG 3’/DAC1-R 5’ATA CAC CGATGA CGT GGC AAT 3’; DAC2-F 5’ATA CATATT CGC AAC CGC GTA 3’/DAC2-R5’ CCA CGT TCGTCC TATAAA GAG 3’; DAC3-F 5’ GCA ACC GCG TAA TAC ATT GTT 3’/DAC3-R 5’AAC GAT TCG GCG TAA GCG AAT 3; DAC4-F 5’ CTC AGA TTG TGG TAC TGGAAC 3’/DAC4-R 5’AAGAAG CTC TCGAGC AAC TGT 3’/DAV2-F 5’ GCT CCGTGGAGT CTT ATT TAG 3’/DAV2-R 5’ATG GTA CAT CGC GTG AAC GAT 3’ specific to DYMV used for the diagnosis of the causal virus infecting dolichos. All the four primers gave positive amplification of targeted DNA fragments indicated the dolichos plant showing yellow mosaic symptoms were infected with DoYMV at Kanpur.