靶向Rab9 GTPase siRNA表达载体的构建和鉴定

【Objective】 To construct small interference RNA (siRNA) expression vector targeting for Rab9 GTPase. 【Methods】 Oligos of 68 base pairs for hairpin RNA targeting for Rab9 GTPase were designed and cloned into the expression vector pSUPER. neo+EGFP to generate the recombinant plasmid pSUPER. neo+EGFP-R1 and pSUPER. neo+EGFP-R2. The recombinant plasmid was confirmed with restriction endonuclease digestion and DNA sequencing. 【Results】 Expression vector was constructed successfully and identified by enzyme digestion analysis. DNA sequence analysis of inserted fragment revealed the same sequences as synthesized siRNA oligonucleotides. 【Conclusion】 The Rab9 GTPase specific small interfering RNA expression vector has been constructed successfully which facilitates the study on relationship between Rab9 GTPase and replication of measles virus.