Diverse CRISPR-Cas complexes require independent translation of small and large subunits from a single gene
2020
Abstract CRISPR-Cas adaptive immune systems provide prokaryotes with defense against viruses by degradation of specific invading nucleic acids. We investigated the previously uncharacterized type I-D interference complex from Synechocystis and revealed it is a genetic and structural hybrid with similarity to both type I and III systems. Surprisingly, formation of the functional complex required internal in-frame translation of small subunits from within the large subunit gene. We further show that internal translation to generate small subunits is widespread across diverse type I-D, I-B and I-C systems, which account for roughly one quarter of CRISPR-Cas systems. Our work reveals the unexpected expansion of protein coding potential from within single cas genes, which has important implications for understanding CRISPR-Cas function and evolution. One Sentence Summary Internal translation of large subunit transcripts drives small subunit synthesis in diverse type I CRISPR-Cas interference complexes
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