Achieving super-resolution fluorescence imaging with focused spot illumination and non-negative inversions (Conference Presentation)

We demonstrate a new method for two-dimensional super-resolution fluorescence imaging. Our method achieves more than 2-fold resolution enhancement over the Rayleigh resolution limit simply with a focused illumination spot and non-negative least-squares inversions, and with practical photon budgets that can be supported by common fluorophores used for biological fluorescence microscopy.