Electrochemical voltammetric behaviors of synthetic dengue virus RNAs at ITO sensing electrode

Abstract An electroconductive indium tin oxide (ITO) electrode was assembled and used as a sensing platform for direct detection of Dengue virus (DENV) RNAs in the phosphate buffer solution at pH 7.4. The voltammetric behaviors of DENV RNAs were examined at different volume ratio with matched and mismatched RNAs. The stability of the DENV RNAs hybrid duplexes and the microenvironment on the electrode surface was found to affect their voltammetric profiles. In this work, the detection limit was estimated to be 2 amol. No labeling, reverse transcription and/or polymerase chain reaction is required for this RNA detection technology. This simple and efficient ITO sensing electrode may be a promising platform in Dengue diagnosis.