A Technique for Investigating the Component of Cantharidin Blisters by Flow Cytometry
2007
Objective: Cantharidimide cause blister.The effect of blister on immunoregulation was investigated.Methods: Cantharidimide was placed on the skin,48h later,the blister was analyzed by flow cytometry.Results: The blister contained 1×106-1×107 cells per ml,most of which were neutrophils,macrophages,dendritic cells(DC),and IL-12 secreted by Th1 cells.Conclusion: There are high concent of DC in the blister,which is differential and induce the secretion of Th1,the activation of T cell.The blister modulate the biological response of patients and is helpful for treatment with infective disease.
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