Validated Liquid Chromatographic Method for Analysis of the Isomers of Latanoprost
2008
Summary Baseline separation of latanoprost and its isomers 15(S)-latanoprost (impurity I) and 5,6-trans-latanoprost (impurity II) has been achieved for the first time by HPLC on an NH2 column with heptane-2-propanol-acetonitrile 93:6:1 (v/v), with addition of a small amount of water, as mobile phase. Validation of the method is described.
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