Production of α-Amylase and Amyloglucosidase by the Fungus Cylindrocladium sp. in Semi-solid State Fermentation

Amylases are very important and have a wide range of applications. Semi-solid state fermentation is increasingly used for the production of enzymes, since it requires simple technology and costs are low. In this regard, it is worth emphasizing the importance of rice as a substrate, due to its readily availability and low cost. This study evaluated the ability of the fungus Cylindrocladium sp . to produce α-amylase and amyloglucosidase. The studies were carried out with the fungus strain Cylindrocladium sp. with a rice-based substrate. The material was kept in an oven at 28 °C for 96 hours and measured every 24 hours. For the quantification of amyloglucosidase enzymes, the DNS reagent method was adopted. The α-amylase activity was determined by measuring the concentration of starch using iodine dosage. Analysis of the data obtained in this study showed that the fungus Cylindrocladium sp. produces α-amylase and amyloglucosidase enzymes; peak production for α-amylase was at 72 hours with 245.02 U/g, and at 92 hours for amyloglucosidase with 73.58 U/g. Through the interpretation of these results it was possible to conclude that the fungus Cylindrocladium sp. produces relevant values of the enzymes α-amylase and amyloglucosidase.