Classification of Benign and Malignant Breast Masses on Mammograms for Large Datasets using Core Vector Machines

AIMS: To improve the stability of protein. BACKGROUND: The stability of protein is of significance to maintain protein function for therapeutical applications. However, it still remains challenging. OBJECTIVE: A general method of preserving protein stability and function was developed using gelatin films. METHOD: Enzymes immobilized onto films composed of gelatin and ethylene glycol (EG) were developed to study their ability to stabilize proteins. beta-glucosidase was selected as a model functional protein. We further assessed the tensile properties, microstructure and crystallization behaviours of the gelatin films. RESULT: Our results indicated that film configurations can preserve the activity of beta-glucosidase under rigour conditions (75% relative humidity and 37 degrees C for 47 days). In both control and films containing 1.8 % beta-glucosidase, tensile strength increased with increased EG content, whilst the elongation at break increased initially and then decreased over time. The presence of beta-glucosidase had a negligible influence on tensile strength and elongation at break. Scanning electron-microscopy (SEM) revealed that with increasing EG content or decreasing enzyme concentrations, more-dense microstructure was observed. CONCLUSION: In conclusion, dry film is a promising candidate to maintain protein stabilization and handling. Other: In addition, the configuration is convenient and cheap, and thus applicable to protein storage and transportation processes in the future.