NMR of Flavoproteins
1999
Flavoproteins are involved in a wide variety of enzymatic reactions like oxidation, reduction and mono-oxygenation. Consequently, flavin cofactors occur in many different forms including, for example, the oxidized semiquinone and reduced states as well as the C4a-(hydro)peroxyflavin form. In flavoprotein research the geometrical and electronic properties of all these different flavin species are of interest. This chapter deals with the use of nuclear magnetic resonance (NMR), a technique enabling the determination of detailed structural and electronic characteristics of flavoproteins and their cofactors.
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