Peroxidase-like reaction by a synergistic inorganic catalyst colloid: a new method for hydrogen peroxide detecting in air samples

A peroxidase-like catalyst of a synergistic inorganic nanocomposite was firstly proposed for the occupational exposure monitoring of H2O2. Nano-zeolite Y (nZY) ion exchange of copper (nZY-Cu) is also used as a peroxidase-like catalyst. In this study, the catalyst component was characterized using X-ray diffraction, BET method, XRF, and scanning electron microscopy. In addition, relative activity was used to show the synergistic peroxidase-like activity of nZY-Cu. Thereafter, the central composite design was applied for the parameter optimization of 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation. However, a method was developed for the TMB-H2O2 reaction using nZY-Cu. The catalytic activity of copper increased in nZY-Cu under the pH of 4.58, with 0.32 mg of catalyst, and TMB concentration of 10.57 μM. Accordingly, a simple and sensitive method was set up for the detection of H2O2 in the air samples, with a linear range of 0.5 to 60 ppm, the detection limit of 0.15 ppm, and a recovery level of 94.13 ± 6.5. The proposed method showed a compatible precision with standard method (OSHAVI-6) according to RSDs.