Identification of Pasteurella multocida Isolated from Rabbit by Using 16S Ribosomal RNA Sequence

Objective To identify Pasteurella multocida using 16S ribosomal RNA gene sequence analysis.Methods Specimens of a suddenly died rabbit were collected.Histopathological examination,culture and isolation of pathogen were carried out.A pairs of primers targeting 16S ribosomal RNA gene conserved region were synthesized.DNA was extracted from isolated bacterial strain,amplified by polymerase chain reaction(PCR),and analyzed 16S rRNA sequence.Results A strain of Pasteurella multocida named PMr0901 was iso1ated and identified from the lung of dead rabbit.Nucleotide sequences were compared with GenBank data base.The BLAST analysis indicated that PMr0901 was the most similar with reported 16S rRNA gene of Pasteurella multocida subsp. multocida str.Pm70 and the similarity more than 99%.The results of drug sensitivity test showed that the isolated bacterial strain was sensitive to amoxicillin/clavulanic acid,ceftriaxone,levofloxacin and tetracycline while was resistant to Penicillin G.Conclusion Molecular-biological approach using the 16S rRNA gene sequence analysis is available for identification of pathogenic bacteria.