Fabrication of a biocompatible and continuous glucose biosensor with the poly(3,4-ethylenedioxythiophene) modified electrode

Abstract In this study, we have explored the potentiality of using GOx/AuNP/PEDOT(BSA)/Pt electrode as an implantable, long-lasting, and sensitive glucose biosensor. We have examined the performance of the electrode for glucose calibration with three electrochemical measurements, such as cyclic voltammetry, linear sweep voltammetry, as well as chronoamperometry, which all exhibited strong linear correlation between resulting signal and glucose concentration. In comparison, linear sweep voltammetry gave the best linear sensitivity, whose average was about 3.124 µA/mM/cm2 within a wide glucose concentration range from 0.416 to 50 mM. Meanwhile, the stability of the electrode was confirmed by a continuous glucose measurement in a 7-day period of time and the deviation was about 5%. The electrode was also biocompatibility according to the ISO109930-1 criterion. Furthermore, we have demonstrated that the oxidation of glucose on the GOx/AuNP/PEDOT(BSA)/Pt electrode was oxygen independent, therefore, it was not only easy to make but also feasible for continuous glucose monitoring systems.