On-line Monitoring: Animal Cell Cultivation in iCELLis™ Fixed-Bed Reactor Using Dielectric Measurements

Artelis/ATMI LifeSciences has developed a novel scalable fixed-bed bioreactor – iCELLisTM based on a proprietary agitation system, a waterfall oxygenation of medium and a packed-bed made up of macroporous carriers. Here we present the use of a permittivity-based biomass probe developed by Fogale to monitors and controls a Vero cell culture producing viruses in such iCELLis fixed-bed. We demonstrate that using a biomass sensor in a fixed-bed bioreactor can follow cell growth and cell lysis (due to viral infection).