Clinical value assessment of chemiluminescence for urine asymmetric dimethylarginine

Objective To explore the clinical application value of chemiluminescence detection of urine asymmetric dimethylarginine (ADMA) in pregnancy-induced hypertension (PIH). Methods Collected the 24 h urine from 60 normal pregnancy women and 72 PIH pregnancy women who were admitted to Huzhou Maternal and Child Health Hospital from May 2014 to April 2015 by the case-control study, Determination of urine ADMA content by chemiluminescence (CLIA) and high performance liquid chromatography (HPLC), the results between two assays were analyzed by the Rank sum test, receiver operating characteristic (ROC) curve and pearson correlation analysis. Results Compared with the normal control group, the urine ADMA concentration in the PIH group was significantly increased by HPLC and CLIA, and the concentration of ADMA by CLIA in the PIH group was 68.18(57.25, 81.55)μmol/L higher than that of the normal control group 30.11(22.69, 42.97)μmol/L(Z=-8.139, P<0.001), and the concentration of ADMA by HPLC in the PIH group by HPLC was 71.11(57.65, 82.89)μmol/L higher than that of the normal control group 28.11(21.06, 42.99)μmol/L(Z=-8.356, P<0.001). The difference was statistically significant.The two methods of urine ADMA concentration were highly positively correlated with PIH blood pressure. The correlation coefficient r values were 0.746 and 0.763, respectively, the P values were 0.007 and 0.008 respectively. Conclusions CLIA can better detect the ADMA concentration in urine of pregnant women with PIH, and has a good clinical diagnosis ability. The ADMA concentration in urine is related to the blood pressure level of PIH.(Chin J Lab Med, 2018, 41: 948-951) Key words: Arginine; Urinalysis; Luminescence; Chromatography, high pressure liquid; Hypertension, pregnancy-induced