Sequencing of PCR Products — Analysis of Factor IX Genes and of Recombination Events in Immunoglobulin Genes

The polymerase chain reaction facilitates DNA sequence analysis due to the simplicity and high sensitivity of the procedure. In many cases the detection of a previously characterized sequence, restriction site or sequence polymorphism is the only information required. This information is most conveniently obtained either by determination of the size of the amplified product on an agarose gel, analysis after restriction enzyme digestion or hybridization using allele-specific oligonucleotides as probes.