Gene vector system containing targeted shading system, preparation and use thereof

The invention relates to a gene delivery system containing a target shielding system and a preparation method and application thereof. The gene delivery system consists of a shielding system with a target ligand, a cation polymer material and plasmid DNA, wherein, the cation polymer material and the plasmid DNA are compounded to form compound particles, and the shielding system with the target ligand is shielded on the surface of the compound through electrostatic effect. The gene delivery system containing the target shielding system can transfer a loaded genic material into cells to realize the expression of the genic material and finish a transfection process, and the targeting of gene transfection can be improved. The preparation method has the advantages that: the target ligand is grafted onto the shielding system; on the one hand, the targeting strategy can not affect the compound ability of a cationic polymer to the DNA, on the other hand, the targeting strategy can also guarantee that the target ligand is extended to the periphery of the gene delivery system so as to improve the combination efficiency of the target ligand and a cell surface receptor. The highest transfection efficiency of the gene delivery system to a HeLa cell is 73 percent, and the toxicity of the cell is small.